Synthesis of Methyl Ester from Microalgae Chlorella sp. TAD Using the In-Situ Transesterification Method

Synthesis of Methyl Ester from Microalgae Chlorella sp. TAD Using the In-Situ Transesterification Method

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Synthesis of methyl ester from Chlorella sp.TAD microalgae was carried out using the in-situ transesterification method.This study aims to determine the methyl ester composition of Chlorella sp.TAD microalgae using the in-situ transesterification method.

The in-situ transesterification method is a modified method that allows extraction and transesterification into methyl ester products in one process Incense simultaneously.The in-situ transesterification process lasted 8 hours, followed by a distillation process to remove the n-hexane content and an oven for 2 hours to evaporate the remaining water.The results of the analysis using GC-MS to determine the chemical content of the methyl ester compound from Chlorella sp.TAD, showed the methyl here ester composition of 7,10-hexadecanoic methyl ester, 8,11,14-docosatrienoic methyl ester, 9-hexadecanoic methyl ester, hexadecanoic methyl ester, heptadecanoic methyl ester, 10-octadecenoic methyl ester, octadecenoic methyl ester, 9,12-octadecadienoic methyl ester, 10-octadecenoic methyl ester, 2-hexyl cyclopropaneoctanoic methyl ester, eicosanoic methyl ester, 10-heptadecen-8-ynoic acid methyl ester, nonahexacontanoic methyl ester, and tetracosanoic methyl ester.